Reply to “Hazardous CaMV promoter?”

Assessment of CaMV-mediated gene silencing and integration of CaMV into GM plants with a 35S RNA promoter.

Four GM plant species (Arabidopsis thaliana, Brassica napus, Nicotiana benthamiana and N. tabacum), each expressing the gene encoding the jellyfish green fluorescent protein (GFP) regulated by the cauliflower mosaic virus (CaMV) 35S RNA promoter, were assessed for the extent of transgene silencing and viral genome integration following infection by CaMV. The first three species are systemic hos...

Semi-quantitative detection of genetically modified grains based on CaMV 35S promoter amplification

The detection and exact quantification of the presence of GMOs (genetically modified organisms, also named as living modified organisms, LMOs) grains has become very important in international commercial transactions, especially from countries producing both types of commodities, GMOs and GMO-free. This makes necessary to check every batch previous delivery to the recipient country. Several PCR...

A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmon

The long-term persistence and activity of a naked plasmid DNA (pGL3-35S) containing a luc gene (reporter gene) controlled by a plant 35S CaMV promoter was studied in Atlantic salmon (Salmo salar L.) after injection. Atlantic salmon (mean weight 70 grams) were injected intramuscularly with 100 μg of plasmid DNA. Blood, different tissues and organs were sampled at different time points up to day ...

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Hazards of Transgenic Plants Containing the Cauliflower Mosaic Viral Promoter Hazards of CaMV Promoter Horizontal Gene Transfer - The Hidden Hazards of Genetic Engineering

Transgenic pollen and baby bees Horizontal gene transfer may spread transgenes to the entire biosphere Genetic engineering is unregulated horizontal gene transfer Artificial vectors enhance horizontal gene transfer What are the hazards of horizontal gene transfer? Potential hazards of horizontal gene transfer from genetic engineering Transgenic DNA may be more likely to transfer horizontally th...